RBP4 ELISA Kit


Assay Characteristics

Format 96 well plate
Specimen serum, plasma, urine
Sample volume 20μl
Working range 0 – 30 μg/l
Incubations 2 x 1 hours, 1 x 15 min
Product code IV3-101E

Key Features

The Invitron RBP4 ELISA is an immunometric assay for the quantitative measurement of retinol binding protein 4 (free RBP4 and complex bound RBP4) in human samples.

Key features of the assay include:
- A new marker used in studies of insulin resistance
- Indicator of chronic liver disease
- Useful for early detection of proteinuria


Protocol

add 100 μl sample to each well
incubate for 1 hour at room temp
wash 5 times and add conjugate
incubate for 1 hour at room temp
wash 5 times and add substrate
incubate for 15 min at room temp
add stop solution and read at 450nm