Adiponectin ELISA Kit


Assay Characteristics

Format 96 well plate
Specimen serum or plasma
Sample volume 25μl
Working range 0 – 90ng/ml
Incubations 2 x 1 hour, 1 x 15 min
Product code IV3-102E

Key Features

The Invitron Adiponectin ELISA is an immunometric assay for the quantitative measurement of adiponectin in human samples.

Key features of the assay include:
- Provides valuable information in studies of metabolic syndrome
- A marker of T2 diabetes, coronary artery disease, atherosclerosis

Protocol

add 100 μl of diluted sample to each well
incubate for 1 hour at room temp
wash 5 times and add conjugate
incubate for 1 hour at room temp
wash 5 times and add substrate
incubate for 15 min at room temp
add stop solution and read at 450nm