Intact Proinsulin ELISA Kit


Assay Characteristics

Format 96 well plate
Specimen plasma (recommended)
or serum
Sample volume 50μl
Working range 0 – 100 pmol/l
Sensitivity 0.3 pmol/l
Specificity Proinsulin 100%
Insulin 0%
C-Peptide 0%
32-33 split proinsulin: 5.6%
Des 31-32 split proins: 1.4%
65-66 split proinsulin: 37%*
Des 64-65 split proins: 63%*
*not present at detectable
levels in human samples
Incubations 1 x 2 hours, 1 x 1 hour
1 x 30 min
Product code IV2-102E

Key Features

The Invitron Intact Proinsulin ELISA is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.

Key features include:

- Very high specificity for 'intact' proinsulin
- Highly specific marker for insulin resistance in type 2 diabetes

- Calibrated against WHO Int'l Standard for Proinsulin (IRP 84/611)

Also available:

Protocol

add 50 μl sample buffer and 50 μl sample to each well
incubate for 2 hours at room temp
wash 3 times and add labelled antibody
incubate for 1 hour at room temp
wash 3 times and add enzyme
incubate for 30 min at room temp
wash 3 times and add substrate
incubate for 20 min at room temp
add stop solution and read at 450nm