Intact Proinsulin Luminescence Assay Kit


Assay Characteristics

Format 96 well plate
Specimen plasma (recommended)
or serum
Sample volume 50μl
Working range 0 – 100 pmol/l
Sensitivity 0.02 pmol/l
Specificity Proinsulin 100%
Insulin 0%
C-Peptide 0%
32-33 split proinsulin: 5.6%
Des 31-32 split proins: 1.4%
65-66 split proinsulin: 37%*
Des 64-65 split proins: 63%*
*not present at detectable
levels in human samples
Incubations 1 x 2 hours, 1 x 1 hour
Product code IV2-002

Key Features

The Invitron Intact Proinsulin Assay is an immunometric assay for the quantitative measurement of intact proinsulin in human samples.
Key features include:
- Easy to use - very simple assay protocol
- High sensitivity detection system
- High specificity for 'intact' proinsulin
- Highly specific marker for insulin resistance in type 2 diabetes
- Calibrated against WHO International Standard (IRP 84/611)

Also available:

Protocol

add 50 μl sample buffer and 50 μl sample to each well
incubate for 2 hours at 37°C
wash 3 times and add 100 μl labelled antibody solution
incubate for 1 hour at 37°C
wash 3 times
read in luminometer