ox-LDL ELISA Kit


Assay Characteristics

Format 96 well plate
Specimen serum or EDTA plasma
Sample volume 10μl
Working range 0 – 250 ng/ml
Incubations 2 x 1 hours, 1 x 15 min
Product code IV3-105E

Key Features

The Invitron ox-LDL ELISA is an immunometric assay for the quantitative measurement of oxidised low-density lipoprotein (ox-LDL) in human samples.

Key features of the assay include:
- Recognises MDA-modified apolipoprotein B 100.


Protocol

add 100 μl of diluted sample to each well
incubate for 1 hour at room temp
wash 5 times and add conjugate
incubate for 1 hour at room temp
wash 5 times and add substrate
incubate for 15 min at room temp
add stop solution and read at 450nm