Total Proinsulin Luminescence Assay Kit


Assay Characteristics

Format 96 well plate
Specimen serum or plasma
Sample volume 25μl
Working range 0 – 250mU/l
Sensitivity 0.25 mU/l
Specificity Proinsulin 100%
Insulin 2.2%
C-Peptide 0%
32-33 split proinsulin: 97%
Des 31-32 split proins: 100%
65-66 split proinsulin: 12%*
Des 64-65 split proins: 1%*
Incubations 1 x 2 hours, 1 x 1 hours
Product code IV2-003

Key Features

The Invitron Total Proinsulin Assay is an immunometric assay for the quantitative measurement of total proinsulin in human samples.

Key features include:
- Easy to use - very simple assay protocol
- High sensitivity detection system
- Calibrated against WHO International Standard (IRP 84/611)


Also available:

Protocol

add 50 μl sample buffer and 50 μl sample to each well
incubate for 2 hours at 37°C
wash 3 times and add 100 μl labelled antibody solution
incubate for 1 hour at 37°C
wash 3 times
read in luminometer